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145 2c11 tonbo  (Cytek Biosciences)


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    Cytek Biosciences 145 2c11 tonbo
    145 2c11 Tonbo, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+anti+cd3e/PE-Cyanine5+Anti-Mouse+CD3e/pm41885414-33-10-11
    Average 93 stars, based on 9 article reviews
    145 2c11 tonbo - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Beneficial effects of sunitinib on tumor microenvironment and immunotherapy targeting death receptor5
    Article Snippet: .. After the blocking, the samples were incubated with the following fluorescence-conjugated mAbs; all the antibodies were from Tonbo Bioscience, USA: FITC or PE anti-CD3e (clone 145- 2C11; 35–0031), allophycocyanin anti-CD4 (clone RM4-5; 20–- 0042), allophycocyanin anti-CD8 (clone 53–6.7; 20–0081), PE anti-Foxp3 (clone 3G3; 50–5773), FITC anti-CD69 (clone H1.2F3; 35–0691), PE anti-IFN-γ (clone XMG1.2; 50–7311), FITC anti-CD11c (clone N418; 35–0114), PE anti-CD80 (clone 16-10A1;50-0801), PE anti-CD86 (clone GL-1; 50–0862). .. For IFN-γ and Foxp3 incubation, Foxp3/Transcription Factor Staining Buffer Kit (TNB-1020-L050, Tonbo Bioscience) was used according to the manufacturer’s instructions.

    Incubation:

    Article Title: Beneficial effects of sunitinib on tumor microenvironment and immunotherapy targeting death receptor5
    Article Snippet: .. After the blocking, the samples were incubated with the following fluorescence-conjugated mAbs; all the antibodies were from Tonbo Bioscience, USA: FITC or PE anti-CD3e (clone 145- 2C11; 35–0031), allophycocyanin anti-CD4 (clone RM4-5; 20–- 0042), allophycocyanin anti-CD8 (clone 53–6.7; 20–0081), PE anti-Foxp3 (clone 3G3; 50–5773), FITC anti-CD69 (clone H1.2F3; 35–0691), PE anti-IFN-γ (clone XMG1.2; 50–7311), FITC anti-CD11c (clone N418; 35–0114), PE anti-CD80 (clone 16-10A1;50-0801), PE anti-CD86 (clone GL-1; 50–0862). .. For IFN-γ and Foxp3 incubation, Foxp3/Transcription Factor Staining Buffer Kit (TNB-1020-L050, Tonbo Bioscience) was used according to the manufacturer’s instructions.

    Fluorescence:

    Article Title: Beneficial effects of sunitinib on tumor microenvironment and immunotherapy targeting death receptor5
    Article Snippet: .. After the blocking, the samples were incubated with the following fluorescence-conjugated mAbs; all the antibodies were from Tonbo Bioscience, USA: FITC or PE anti-CD3e (clone 145- 2C11; 35–0031), allophycocyanin anti-CD4 (clone RM4-5; 20–- 0042), allophycocyanin anti-CD8 (clone 53–6.7; 20–0081), PE anti-Foxp3 (clone 3G3; 50–5773), FITC anti-CD69 (clone H1.2F3; 35–0691), PE anti-IFN-γ (clone XMG1.2; 50–7311), FITC anti-CD11c (clone N418; 35–0114), PE anti-CD80 (clone 16-10A1;50-0801), PE anti-CD86 (clone GL-1; 50–0862). .. For IFN-γ and Foxp3 incubation, Foxp3/Transcription Factor Staining Buffer Kit (TNB-1020-L050, Tonbo Bioscience) was used according to the manufacturer’s instructions.



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    Image Search Results


    USP8 deletion impairs osteoclast development and bone resorption activity. ( A – E ) Usp8 fl/fl and Usp8 Ctsk BMMs were cultured under osteoclast differentiation conditions for 6–8 days. mRNA levels of Usp8 ( n = 8, A) and osteoclast marker genes ( n = 8, E) were assessed by qRT-PCR. Osteoclast differentiation was assessed by measuring TRAP staining and activity ( n = 15, B and D) and osteoclast activity ( n = 5, C) was assessed using resorption pit assays. ( F – I ) RAW 264.7 cells were transduced with lentiviruses expressing control shRNA (shCtrl) or Usp8 shRNA (shUSP8). After puromycin selection, cells will be cultured under osteoclast differentiation conditions for 6–8 days. mRNA levels of Usp8 ( n = 6, F) and osteoclast marker genes ( n = 5, I) were assessed by qRT-PCR. Alternatively, cells were stained with TRAP (G, left) and numbers of TRAP-positive multi-nucleated cells and nuclei ( n = 7–10, G, middle and right) and TRAP activity in the supernatant of cell culture ( n = 6, H) were quantified. Scale bars: 100 µm (B, C, G). ( J ) Bone marrow cells were isolated from 8-week-old Usp8 fl/fl and Usp8 Ctsk femurs and tibias and then, subjected to flow cytometric analysis to identify osteoclast precursors (OCPs). Representative dot plots (left) and quantification (right) are displayed to show the frequency of CD11b −/lo Ly6C hi CD3 − Ter119 − B220 − OCPs ( n = 5). Values represent mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by a two-tailed unpaired Student’s t-test for comparing two groups. Representative images of ten replicates are displayed ( B , C , G )

    Journal: Cellular and Molecular Life Sciences: CMLS

    Article Title: USP8-mediated mitochondrial regulation in osteoclasts is essential for skeletal development

    doi: 10.1007/s00018-025-06012-0

    Figure Lengend Snippet: USP8 deletion impairs osteoclast development and bone resorption activity. ( A – E ) Usp8 fl/fl and Usp8 Ctsk BMMs were cultured under osteoclast differentiation conditions for 6–8 days. mRNA levels of Usp8 ( n = 8, A) and osteoclast marker genes ( n = 8, E) were assessed by qRT-PCR. Osteoclast differentiation was assessed by measuring TRAP staining and activity ( n = 15, B and D) and osteoclast activity ( n = 5, C) was assessed using resorption pit assays. ( F – I ) RAW 264.7 cells were transduced with lentiviruses expressing control shRNA (shCtrl) or Usp8 shRNA (shUSP8). After puromycin selection, cells will be cultured under osteoclast differentiation conditions for 6–8 days. mRNA levels of Usp8 ( n = 6, F) and osteoclast marker genes ( n = 5, I) were assessed by qRT-PCR. Alternatively, cells were stained with TRAP (G, left) and numbers of TRAP-positive multi-nucleated cells and nuclei ( n = 7–10, G, middle and right) and TRAP activity in the supernatant of cell culture ( n = 6, H) were quantified. Scale bars: 100 µm (B, C, G). ( J ) Bone marrow cells were isolated from 8-week-old Usp8 fl/fl and Usp8 Ctsk femurs and tibias and then, subjected to flow cytometric analysis to identify osteoclast precursors (OCPs). Representative dot plots (left) and quantification (right) are displayed to show the frequency of CD11b −/lo Ly6C hi CD3 − Ter119 − B220 − OCPs ( n = 5). Values represent mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 by a two-tailed unpaired Student’s t-test for comparing two groups. Representative images of ten replicates are displayed ( B , C , G )

    Article Snippet: Cells were washed twice with cold fluorescence-activated cell sorting (FACS) buffer, incubated with Fc blocking buffer (BD Biosciences, 564765) for 15 min at 4 °C, and treated with antibody cocktail including CD3 (Tonbo Biosciences, 50–0031), CD45R/B220 (Tonbo Biosciences, 65–0452), Ter119 (BioLegend, 116233), CD11b (Tonbo Biosciences, 20–0112), and Ly6C (BioLegend, 128017) in cold FACS buffer.

    Techniques: Activity Assay, Cell Culture, Marker, Quantitative RT-PCR, Staining, Transduction, Expressing, Control, shRNA, Selection, Isolation, Two Tailed Test